aconitase 2  (Boster Bio)

Journal: International Journal of Nanomedicine

Article Title: Yttrium Oxide Nanoparticles Affect Both Cognitive and Memory Function by Disrupting Copper Output in Neuronal Cells in a Rat Model

doi: 10.2147/IJN.S515951

Figure Lengend Snippet: Y 2 O 3 NPs induce cuproptosis in the rat cerebral cortex. ( A ) IHC staining of DLAT and Fe-S cluster proteins (FDX1, ACO2, LIAS, and SDHB) in the rat cerebral cortex after exposure to Y 2 O 3 NPs. ( B ) Western blot and quantification of DLAT and Fe-S cluster proteins (POLD1, FDX1, ACO2, LIAS, and SDHB) in rat cerebral cortex cells after exposure to Y 2 O 3 NPs. The results are shown as the means ± SDs. * P <0.05 and ** P <0.01.

Article Snippet: The expression of dihydrolipoamide S-acetyltransferase (DLAT), iron-sulfur (Fe-S) cluster proteins, and ATP7A was evaluated using antibodies against recombinant ferredoxin 1 (FDX1) (1:200, ABclonal, China), DLAT (1:100, Proteintech, USA), aconitase 2 (ACO2) (1:100, Proteintech, USA), lipoyl synthase (LIAS) (1:100, Proteintech, USA), succinate dehydrogenase complex, subunit B (SDHB) (1:200, Proteintech, USA) and ATP7A (1:100, Abmart, China).

Techniques: Immunohistochemistry, Western Blot

Journal: International Journal of Nanomedicine

Article Title: Yttrium Oxide Nanoparticles Affect Both Cognitive and Memory Function by Disrupting Copper Output in Neuronal Cells in a Rat Model

doi: 10.2147/IJN.S515951

Figure Lengend Snippet: Y 2 O 3 NPs induce cuproptosis in neuronal cells in vitro. ( A ) Western blot analysis and quantification of DLAT and Fe-S cluster proteins (FDX1, POLD1, ACO2, LIAS, and SDHB) in PC12 cells after treatment with Y 2 O 3 NPs or ES. ( B ) IF analysis of DLAT and Fe‒S cluster proteins (FDX1, ACO2, and LIAS) in PC12 cells after treatment with Y 2 O 3 NPs or ES. The results are shown as the means ± SDs. * P <0.05, ** P <0.01 and *** P <0.001.

Article Snippet: The expression of dihydrolipoamide S-acetyltransferase (DLAT), iron-sulfur (Fe-S) cluster proteins, and ATP7A was evaluated using antibodies against recombinant ferredoxin 1 (FDX1) (1:200, ABclonal, China), DLAT (1:100, Proteintech, USA), aconitase 2 (ACO2) (1:100, Proteintech, USA), lipoyl synthase (LIAS) (1:100, Proteintech, USA), succinate dehydrogenase complex, subunit B (SDHB) (1:200, Proteintech, USA) and ATP7A (1:100, Abmart, China).

Techniques: In Vitro, Western Blot

Journal: International Journal of Nanomedicine

Article Title: Yttrium Oxide Nanoparticles Affect Both Cognitive and Memory Function by Disrupting Copper Output in Neuronal Cells in a Rat Model

doi: 10.2147/IJN.S515951

Figure Lengend Snippet: ATP7A mediates copper output disorder induced by Y 2 O 3 NPs. ( A ) Viability of PC12 cells after ATP7A overexpression. ( B ) Cu content in PC12 cells after ATP7A overexpression. ( C ) Western blot and quantification analysis of ATP7A, DLAT and Fe-S cluster proteins (FDX1, POLD1, ACO2, LIAS, and SDHB) in PC12 cells after ATP7A overexpression. The results are shown as the means ± SDs. * P <0.05, ** P <0.01, and *** P <0.001.

Article Snippet: The expression of dihydrolipoamide S-acetyltransferase (DLAT), iron-sulfur (Fe-S) cluster proteins, and ATP7A was evaluated using antibodies against recombinant ferredoxin 1 (FDX1) (1:200, ABclonal, China), DLAT (1:100, Proteintech, USA), aconitase 2 (ACO2) (1:100, Proteintech, USA), lipoyl synthase (LIAS) (1:100, Proteintech, USA), succinate dehydrogenase complex, subunit B (SDHB) (1:200, Proteintech, USA) and ATP7A (1:100, Abmart, China).

Techniques: Over Expression, Western Blot